PN-III-P1-1.1-TE-2019-1179
PROJECT CODE: PN-III-P1-1.1-TE-2019-1179, contract number TE 94 ⁄ 2020
PROJECT TITLE:
New sol-gel-magnetic biocatalysts used for the enzimatic hydrolysis of lignocellulosic biomass
Noi biocatalizatori sol-gel-magnetic utilizati pentru hidroliza enzimatică a biomasei lignocelulozice
ACRONYM: BIOCATLIGNOCELL
PROJECT COORDINATOR: Politehnica University Timişoara
PROJECT DIRECTOR: Lecturer dr. eng. Ana Cristina Paul

BUDGET: 431.900 RON (Public budget)
START TIME: 15/09/2020
END TIME: 14/09/2022

PROJECT LAYOUT
The major cause of environmental pollution is due to emissions generated by burning of fossil fuels. The known crude oil reserves are going to disappear in short time and the oil crisis in recent years, together with the rising of air pollution levels has shown the need for the replacement of fossil fuels with cleaner biofuels, obtained by processing a range of organic renewable raw materials. The first step in conversion of lignocellulosic biomass to bioethanol is the pretreatment for the release of cellulose from the network formed with lignin and to increase the yield of fermentable sugars. There are many methods of pretreatment, but they are energy consumable and pollute the environment.
In this sense the project proposes an innovative approach on studies regarding biomass pretreatment and enzymatic hydrolysis of cellulose in an integrated system that can improve the exploitation of biomass components and the reuse of the biocatalyst. It is desired to provide novel biocatalysts, immobilized cellulases customized by new sol-gel entrapment techniques, used in the hydrolysis of certain types of lignocellulosic biomass. By immobilization, stability and reusability of cellulases are significantly improved, a key issue for increasing the amount of fermentable sugars and to reduce process costs.


PROJECT TEAM:
Project leader: Lecturer dr. eng. Ana Cristina Paul
Postdoctoral researchers:
Lecturer dr. eng. Gerlinde Rusu
Lecturer DVM Simona Marc
RA chem. Corina Vasilescu (PhD student)

OBJECTIVE:
The main objective of the project is the obtaining of new immobilized enzymatic biocatalysts, customized by new sol-gel entrapment techniques, used for the hydrolysis of certain types of lignocellulosic biomass.

STAGES
Stage 1 (2020, 4 months)- Pretreatment of lignocellulosic biomass using unconventional methods.


Stage 2 (2021, 12 months)- Immobilization of cellulases using new sol-gel entrapment technics and structural and operational characterization of the obtained biocatalysts.


Stage 3 (2022, 8 months)- Enzymatic hydrolysis of the cellulose from biomass and biocatalyst reuse.


DELIVERABLES:
Stage 1
Lignocellulosic biomass pretreatment protocol.
Optimized lignocellulosic biomass pretreatment protocol.
Stage 1 report.


Stage 2
Immobilization protocol for cellulases.
Optimized immobilization protocol for cellulases using sol-gel technique.
Multigram quantities of biocatalysts with well-defined characteristics.
Stage 2 report.
Abstract for one presentation at scientific conference.
A scientific article submitted for publication / accepted in the journal with impact factor indexed in the Web of Science database.

Stage 3
Optimized protocol for enzymatic hydrolysis of lignocellulosic materials.
Technological laboratory process for enzymatic hydrolysis of cellulose from pre-treated biomass.
Stage 3 report.
Final report.
Abstract for one presentation at scientific conference.
A scientific article submitted for publication / accepted in the journal with impact factor indexed in the Web of Science database.

RESULTS 2021

Publications:

  1. C. Vasilescu, C. Paul, S. Marc, I. Hulka, F. Péter, Sustainable development of a sol-gel entrapped biocatalyst for flavor ester synthesis, Journal of Cleaner Production, 2021, under revision.

Conferences:

  1. C. Vasilescu, S. Marc, S. Liga, C. Paul, Comparative study and optimization of lignocellulosic biomass pretreatment methods, 15th International Symposium on Biocatalysis and Biotransformations (Biotrans 2021 - Online), 19-22 July 2021, Graz, Austria.
  2. C. Vasilescu, C. Paul, F. Péter, Tailoring the sol-gel entrapment method of enzymes by design of experiments for solvent-free ester synthesis, 15th International Symposium on Biocatalysis and Biotransformations (Biotrans 2021 - Online), 19-22 July 2021, Graz, Austria.
  3. C. Vasilescu, S. Marc, G. Scheuleac, C. Paul, Sol-gel entrapment of cellulases for the enzymatic hydrolysis of lignocellulosic biomass to fermentable sugars, 13th European Congress of Chemical Engineering and 6th European Congress of Applied Biotechnology (ECCE&ECAB 2021, virtual event), 20-23 September 2021, Berlin, Germany.
  4. I. Miloș. M. Gheorghe, C. Vasilescu, F. Péter, C. Paul, Batch and continuous flow aroma ester synthesis by sol-gel entrapped Candida antarctica lipase B, The 13th online edition of symposium with international participation, New trends and strategies in the chemistry of advanced materials with relevance in biological systems, technique and environmental protection, 7-8 October 2021, Timisoara, Romania.

 

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